Pre-harvest sprouting (PHS) is one of the most important problems in wheat production especially in environments characterized by the occurrence of rainfall and high humidity prior to harvest as it significantly reduces grain yield and quality. Given that pre-harvest sprouting tolerance is a quantitatively inherited trait, identifying quantitative trait loci (QTL) that control PHS is key to facilitate marker-assisted breeding of PHS resistant cultivars. This study is therefore aimed at identifying QTLs and genes for PHS resistance using a doubled haploid (DH) population of 330 lines derived from a cross between ‘Roblin’ (PHS susceptible cultivar) and RL4137 (PHS resistant breeding line). To this end, PHS phenotype of the DH population was examined based on germination index values obtained from four field trials over two years while the population was genotyped using a 90 K Infinium iSelect Custom Wheat Beadchip. The genotypic data were used to develop a high-density linkage map consisting of 8751 SNP markers and all the SNP markers were mapped across the 21 chromosomes, resulting in 34 linkage groups with total map size of 2343.8 cM and map density of 0.3 cM/marker. Inclusive composite interval mapping (ICIM) was used to identify QTL for PHS resistance. Seven prominent QTLs were detected on linkage groups 1A, 1D, 4A.1, 4B, 5B, 6B.1 and 7A over four environments. Of these, two stable QTLs were identified in more than two environments, a QTL on linkage group 4A.1 and another QTL on linkage group 6B.1. The QTL on linkage group 4A.1 was found to be the most consistent across all trials and explained 40% to 50% of phenotypic variation, indicating the importance of this QTL in regulating PHS resistance. Markers tightly linked with this QTL will be critical in facilitating further studies for fine mapping, candidate gene discovery and marker assisted selection of PHS resistant cultivars.