Leaf rust and powdery mildew, caused by Puccinia triticina and Blumeria graminis f. sp. tritici, respectively, are widespread fungal diseases of wheat (T. aestivum L.) that can cause yield loss in susceptible cultivars by decreasing kernel number and reducing kernel weight. The genetic determinants of the two resistances have been investigated by QTL analysis in a bread wheat RIL population (‘Victo’ x ‘Spada’), for which a high-resolution genetic map, based on 90k Infinium Illumina SNPs, is available. The analysis of leaf rust resistance allowed the identification of one major gene on the long arm of chromosome 5D (QLr-gb.5D) that explains about 59% of phenotypic variance and an additional minor gene was located on chromosome 2B (QLr-gb.2B), explaining about 16% of total phenotypic variation. A single dominant gene for powdery mildew resistance was located on the long arm of chromosome 7A (QPm-gb.7A) explaining 90% of total phenotypic variation. For all regions identified, Victo contributed the alleles with the positive effect. The major QTLs identified in the present study, QLr-gb.5D and QPm-gb.7A, were projected on the T. aestivum reference genome sequence (cv. ‘Chinese Spring’) to define their physical interval. The genetic confidence interval (CI) of the leaf rust locus was of 2.15 cM corresponding to 2.46 Mbp, whilst the powdery mildew region on chromosome 7A (CI: 1.4 cM), extends for 6.34 Mbp. The physical regions underlined by the QTLs were inspected to identify putative candidate genes. This research permitted us to highlight 11 and 7 open reading frames related to disease resistance genes for leaf rust and powdery mildew regions, respectively. The results obtained in this study allowed the identification of resistance loci and candidates for leaf rust and powdery mildew resistance in a hexaploid wheat genetic background. The cv. Victo acted as donor of the resistance loci towards both pathogens suggesting its usefulness in breeding program.