Canada Grain protein concentration (GPC) is an important trait in durum cultivar development as a major determinant in the nutritional value of grain and end-use product quality. Unfortunately, GPC is negatively associated with grain yield. To characterize the quantitative trait loci (QTL) controlling GPC in Canadian durum wheat, a doubled haploid (DH) population, Pelissier/ Strongfield, consisting of 162 lines segregating for GPC was studied. The population was grown in the field over five years and protein concentration of grain samples was measured on whole grain samples using NIR. A genetic map was constructed using data from the 90K Illumina iSelect Wheat Infinium SNP (single nucleotide polymorphism) array.QTL contributing to protein concentration were detected on chromosome 1B, 2B, 3A, 5B, 7A and 7B. One major QTL on chromosome 3A was detected across all environments accounting for 9-25% of the phenotypic variance, with the favorable allele derived from Pelissier. Another major QTL expressed in four environments, was located on chromosome 7A and explained 7-15% of the phenotypic variance, with the beneficial allele derived from Strongfield. The most closely associated SNP markers can be assayed with Kompetitive Allele Specific PCR (KASP) technique for efficient and high throughput marker assisted breeding for GPC in durum. The QTL that expressed stably across environments should be valuable in maintaining protein concentration under selection for higher grain yield.