Bread wheat is an important food crop which plays a key role in the global food supply. Improvement of wheat end-use quality, agronomic performance and disease resistance is an important goal for wheat breeders. The 1RS.1BL translocation has been extensively utilized in wheat breeding program across the world for multiple disease resistance, abiotic stress tolerance and higher grain yield. However, the presence of Sec-1 locus on 1RS arm of rye, encoding rye storage protein (secalin) is responsible for dough stickiness. The absence of Glu-B3/Gli-B1 on 1BS further reduces the bread making quality. Increased root biomass and higher grain yield are associated with 1RS.1BL translocation as one of the major QTL for root traits mapped on 1RS is linked to Pm8 in the distal region of 1RS arm. In the present study a 1RS.1BL recombinant line MA1 in Pavon (without Sec-1 and with Glu-B3/Gli-B1) was used as the donor parent for introgression into elite wheat cultivars (HD2967+Lr57+Yr40, UP2338 and HS240) using marker assisted backcross breeding (MABB). Three BC2F5 populations (without Sec-1; with Glu-B3/Gli-B1 & Pm8) have been developed and their detailed analysis for micro SDS sedimentation, high molecular weight glutenin subunits (HMWGS), bread making quality and root trait parameters has been done. The derivatives with comparable grain yield with the recipient cultivars and improved bread making quality have been developed.