Application of doubled haploid (DH) technology can speed up wheat cultivar development. Colchicine-mediated chromosome doubling is the key step in producing doubled haploids. Colchicine is highly toxic to humans and animals and its use may cause potential environmental pollution. Genes for controlling unreduced gametes have been identified and could be used for doubled haploid production without the use of colchicine. The objective of this study was to validate the function of the unreduced gamete genes derived from Triticum turgidum ssp turgidum AS2255. A Chinese cultivar “Jianongmai 809” with ID Z1586 was crossed with a wheat line Z844 that was considered having unreduced gamete gene(s). F1 plants of Z844 / Z1586 and the two parents were used for DH production using the corn pollination method without colchicine treatment. A total of 929 Z844, 604 Z1586 and 1628 F1 florets were emasculated and pollinated with corn pollen from which, 131 (14.1%), 58 (9.6%) and 96 (6.2%) haploid embryos were obtained, respectively. Ultimately, 11 Z1586, 10 Z844 and 10 F1 adult haploid plants were produced. One tiller on one adult F1 haploid plant Z-Z-6 produced 37 seeds. The seeds harvested from plant Z-Z-6 were likely the result of the union of unreduced gametes. Further studies will be needed to better understand the expression of the unreduced gamete trait.