According to results from an Australian study, some wheat varieties were more prone to damage from certain herbicides than others. They tested 19 wheat varieties in their studies, and yield reductions as high as 41% were recorded in some varieties with certain treatments. The variance in tolerance can be ascribed to differences in morphological characteristics, physiological processes and/or the tempo of internal ear development. In addition, the applied herbicide rate, environmental conditions during herbicide application and growth stage of the crop may also have an impact on tolerance levels among different varieties. In South Africa, there are currently 11 commercially available dryland spring wheat cultivars (for planting in the Western Cape) in the ARC-Small Grain National Cultivar Evaluation trials. To date, no herbicide tolerance studies have been performed on these cultivars, leaving producers uninformed about the herbicide tolerance status of these cultivars. A research project, conducted by ARC-Small Grain, was conducted to determine the phytotoxicity of herbicides to six Western Cape cultivars. These cultivars were planted in two regions, i.e, Rûens (Tygerhoek, 2015 – 2016) and Swartland (Moorreesburg, 2015; Wellington, 2016). The trials were treated with six herbicide treatments (including the control), each containing different herbicides or combinations thereof. Results obtained revealed that no herbicide treatment performed equally well in both areas for the same cultivar while clear differences were observed between the different cultivars. Overall, the two herbicides that led to the lowest yields over both areas in 2015 and 2016 were herbicides with the active ingredients pyroxsulam and iodosulforon-methyl-sodium & mesosulfuron-methyl & safener. It was interesting to note that iodosulforon-methyl-sodium & mesosulfuron-methyl & safener showed the highest yields in two cultivars in the Swartland and Rûens, respectively, confirming the suspicion that herbicides act differently in different areas and on different cultivars. The results from this research will inform wheat producers about the possible phytotoxic effect that some herbicides may have on certain wheat cultivars. Producers must be able to balance the risk of crop damage due to phytoxicity against the potential yield loss as a result of weed competition.