The stripe, leaf, and stem rust diseases of wheat caused by Puccinia striiformis f. sp. Tritici (Pst), P. triticina (Pt), and P. graminis f. sp tritici (Pgt) are the most devastating diseases of wheat worldwide. Growing resistant cultivars is the preferred control strategy particularly in low yielding cropping systems where fungicide application is not affordable. Emergence of new virulent races in pathogen populations overcoming the used resistant genes, call for more efforts to identify new sources of resistance for use in the breeding programs. Genetic resources, mainly landraces are key in supplying new genes of resistance to rusts. In the present study, genome-wide association studies (GWAS) with a mixed linear model was implemented in characterization of resistance to stripe, leaf and stem rust in 2300 bread wheat landrace accessions. These landraces were selected from ICARDA genebank and were originally collected from Iran, Turkey and Afghanistan. Phenotypic evaluation of adult-plant resistance to the three rusts was conducted at the “precision wheat rust phenotyping platform, Regional Cereal Rust Research Center in Izmir Turkey. For field assessments, stripe rust race “Warrior”, the local race of stem rust “TKTTF”, and mixture of local races of leaf rust were used in artificial inoculations of the accessions. The panel was genotyped with a wheat DArtseq single nucleotide polymorphism (SNP) array of which 23K SNPs were used in GWAS analysis. The Principal Coordinate Analysis (PCA) grouped accessions in 3 groups. Using Mixed Linear Model (MLM) implemented in the R (GAPIT), 40 SNPs located on chromosomes 1B, 2A, 2D, 3D, 4D, 5A, 5B, 7A and unknown regions were identified to be associated with leaf rust resistance, 39 SNPs located on chromosomes 1A, 1B, 2D, 3A, 3B, 4A, 5A, 6D, 7A, 7D and unknown regions in the genome were identified to be associated with resistance to stripe rust race “Warrior”, and 14 SNPs located on 2A, 2B, 3A, 4A, 6B, 6D, 7B, 7D and unknown regions were identified to be associated to resistance to stem rust race TKTTF. In present study potentially, novel sources of resistance to the three rust diseases were identified however further investigations using different races of Pst, Pt, and Pgt (including Ug99) are on-going for precise characterization of seedling and adult-plant resistance.