Fusarium head blight (FHB or scab) caused by Fusarium spp. is a destructive disease of wheat. Host resistance coupled with other integrated pest management practices is considered the best approach to control FHB. In an effort to identify novel sources of FHB resistance, four thousand accessions of Triticum aestivum from Plant Gene Resource of Canada (PGRC) were evaluated in a field FHB nursery for two seasons; 400 lines with the greatest resistance were selected for genome-wide association study (GWAS) to identify novel FHB alleles/or QTLs. GWAS identified QTLs for FHB incidence on 1B, 2B, 7A and 7B, and for FHB severity on chromosomes 1A, 1B, 2B, 3A, 3D, 4A, 5A, 5B, and 6A. In addition to the PGRC panel, 412 lines were evaluated from a synthetic hexaploid wheat association mapping (SHW AM) panel, which were created by crossing durum wheat (AABB) and Aegilops tauschii (DD) at the International Wheat Research Centre in Mexico (CIMMYT). Thirty-eight SHW consistently showed low incidence and severity (comparable to the resistant check Sumai3) across biological replications and multi-year testing in the field. All 38 lines also had low DON accumulation. Greenhouse evaluation of Type II resistance of these 38 lines identified 2 lines with very strong resistance. These lines will be useful in the development of FHB-resistant wheat germplasm and populations for discovery of novel FHB resistance. GWAS analysis of the SHW AM, identified QTLs for FHB incidence and severity in 2016 and 2017. Seven high priority QTLs, on chromosomes 2A, 2D, 3B, 5A, 5D, 7B and 7D were selected, and Kompetitive allele specific PCR (KASP) markers flanking these QTLs developed for use in marker assisted selection (MAS).