Wheat (Triticum aestivum L.) faces stress due to drought events at different phenological stages. Limited water at heading, anthesis and grain filling period is considered more critical for yield penalty. However, soil moisture limitation at germination and early seedling stage is often equally critical as it potentially lead to poor plant establishment, ultimately resulting in low grain yield. While breeding for drought tolerance, the common practice is to consider traits at vegetative or latter growth stages for evaluation and selection. In order to demonstrate the high throughput evaluation of wheat genotypes at the early stage, we phenotyped a panel of 318 spring wheat genotypes for seedling traits under semi-controlled environments. Tenday old plants from the genotypes in the panel were phenotyped for seedling root traits in a hydroponic growth assembly, using germination pouches at growth room conditions. On the 11th day images of the roots were taken and analyzed for different traits such as root surface area, root diameter, root tip count and root density using image analysis. The genotypes in the panel varied significantly for these traits. The panel was also phenotyped for coleoptile length and other seedling traits such as root length, and root and shoot dry biomass ratio of 7-day old plants, under GA3 treated and control conditions. Genotypes differed significantly for all the traits evaluated under both treated and controlled conditions. In addition, the panel has been genotyped by using genotyping-by-sequencing (GBS) method. We will be sharing the results from phenotypic and association studies with specific focus on the applicability and implications of high throughput phenotyping methods and genomic selection for these traits, which can potentially be employed by the wheat breeders to increase the efficiency in breeding drought tolerant wheat varieties.