Wheat production is globally affected by stripe rust disease. Stripe rust disease can effectively be controlled by the deployment of multiple resistance genes that confer durable resistance. One of the most effective strategies to incorporate resistance genes is by marker assisted gene pyramiding. The aim of this study was to pyramid Yr15 and Yr36 stripe rust resistance genes by utilizing molecular markers in wheat variety PBW550 and phenotypic evaluation of the pyramided lines. The study was based on BC2F4 introgression lines Yr15 from donor parent Avocet+Yr15 and BC2F4 Yr36/Gpc-B1 from donor parent Glupro in the background of PBW550. Eleven lines of Yr36 introgression were crossed with 5 lines of Yr15introgression lines to pyramid the two genes. 55 F2 populations were developed and narrowed down to 5 populations carrying 559 plants which was further reduced to 138 plants on the basis of plant type, rust resistance and grain type. Molecular markers (Xgwm498 for Yr15 and Xucw130 and Xucw108 for profiling Yr36) revealed 10 homozygous plants for Yr15and Yr36/Gpc-B1genes. Parental polymorphism survey was carried out among the parents by using 240 SSR markers out of which sixty two markers (25.8%) showed parental polymorphism. Background molecular marker profiling of ten pyramided plants showed the recovery of PBW550 genome from 82.2% to a maximum of 91.9%. Agronomic and quality trait evaluation of pyramided lines showed that they were at par or better thanPBW550.