According to the World Health Organization, more than 2 billion people across the globe are suffering from dietary deficiency of iron. This problem is worse in developing nations and severely affects children and pregnant women. Bread wheat (AABBDD genomes) is an important staple crop and most of the flour milled from commercial wheat varieties have suboptimal levels of iron. Bread wheat has a polyploid genome, which makes it even more difficult to make any genetic changes. It was previously reported that the wild non-progenitor species of wheat Aegilops kotschyi (UUSS genome) has a high grain mineral content and hence, could be utilized for wheat biofortification programs. WheatAegilops interspecific derivatives with high grain iron and zinc were developed by using various conventional and molecular breeding techniques, such as pollen and seed radiation hybridization, and making use of ph1b mutant and Mono5B lines for precise transfers without linkage drag. Several selected derivative lines showed introgression of chromosome 2U/2S from Aegilops kotschyi conferring high grain mineral micronutrients in wheat and without any yield penalty. We are also investigating Watkins wheat landraces, collected from different parts of the world, and being maintained at the John Innes Centre UK. Biparental populations of Watkins lines crossed with Paragon are currently being studied to find candidate gene(s) for high mineral content. These gene(s) will be further utilized in the wheat biofortification programs with the goal of higher bioavailable iron in the edible part of wheat grain, without any concentration effect and yield penalty.