Fusarium head blight (FHB) resistance QTLs have been identified in a number of wheat germplasms. However, not all of them have been evaluated for in breeding programs. Particularly, those QTLs in many indigenous germplasms have hardly been used in breeding due to poor agronomic performance of the donor parents. In previous studies, we determined two major QTLs, Fhb1 and Fhb2, for type II resistance, and two major QTLs, Fhb4 and Fhb5, for type I resistance in Chinese wheat landrace Wangshuibai. In this study, we first transferred these QTLs, in different combinations, to susceptible lines PH691 and Mianyang 99-323 through marker-assisted selection (MAS). Evaluation of the obtained lines in the field for two planting seasons showed that Fhb1 and Fhb2 effectively increased type II resistance, and Fhb4 and Fhb5 effectively increased type I resistance in both backgrounds. These QTLs functioned additively and the effect of Fhb1 was larger than Fhb2. Using a nearisogenic line of the PH691 background that carried all the four QTLs as the donor parent, these QTLs were transferred into other nine elite wheat cultivars or lines through backcrossing for three generations. The effects of these QTLs on FHB resistance were again substantiated in field trials, though varied in different genetic backgrounds.