Wheat blast (WB), caused by Magnaporthe oryzae Triticum pathotype (MoT), is currently one of the most destructive diseases threatening wheat production worldwide. WB is endemic in South America, first reported in 1985 in Brazil and subsequently spread to Bolivia (1996), Paraguay (2002), northern Argentina (2007) and Bangladesh (2016). Despite this, WB has not been detected in wheat in Uruguay, probably due to unfavorable environmental conditions for the infection and development of the pathogen during the crop cycle. However, MoT has been reported in Uruguay in rice, ryegrass, and barley. To date, the alien fragment 2N"S transferred from the wheat relative Aegilops ventricosaholds the only useful source of WB resistance. The presence of the 2N"S fragment in the Uruguayan wheat cultivars and breeding germplasm might be one of the reasons why WB has not been detected in Uruguay yet. We evaluated the presence of the 2N"S fragment in 3977 wheat materials that are part of the germplasm from INIA (National Agricultural Research Institute) breeding program. The plant material included, advanced wheat breeding lines and pre-breeding germplasm, CIMMYT germplasm and materials from other global breeding programs introgressed into INIA adapted materials and cultivars bred abroad and released in Uruguay. In order to predict the presence of 2N"S fragment, we used skim-sequencing and a bioinformatic customized pipeline developed in house. Only 495 lines (14.2% of the total) resulted positive for the presence of 2N"S, suggesting that the alien fragment is present in a low proportion. Additionally, we checked the 2N"S presence for some widely cultivated varieties in Uruguay in 2018, which accounted for c.a. 56,000 ha (one-fourth of the total wheat area). Interestingly, c.a. 30% of the area was grown with resistant cultivars (positive for 2N"S), suggesting that resistance might have helped to prevent WB establishment. Our results also suggest that INIA wheat breeding program should start an introgression plan for the 2N"S in order to prevent a future WB outbreak.