DETERMINING THE EFFECT OF ALIEN CHROMOSOMES ON RATE OF RECOMBINATION IN DURUM WHEAT Abstract uri icon

abstract

  • Recombination rates along wheat chromosomes can vary significantly from region to region. Recombination hotspots are common in the telomeric regions of the wheat chromosomes while centromeric regions are largely void of recombination. This has consequences for plant breeders who ideally would like maximum recombination among all genes in order to provide maximum variability. Disturbances in meiosis can lead to changes in the rate and distribution of chiasmata within paired homologues. In this project, we examined the influence of alien chromosomes on recombination in two tetraploid wheat backgrounds. The material was developed from Langdon triticale (AABBRR; Langdon X Rye-13) along with two tetraploid (AABB) durum wheats, Langdon and W9262-260D3. Two crosses were made in which the first cross was the normal durum wheat cross (Langdon X W9262-260D3) and the second was between the hexaploid triticale and durum wheat (Langdon triticale X W9262-260D3) so that rye univalents would be present during the F1 meiosis. The genetic mapping of 200 samples from each cross was performed using the genotypic data from the wheat 90K iSelect array. We are currently comparing genetic maps from both of these crosses. Our results will show the impact of rye univalents on genetic recombination in the wheat homologous chromosomes. If successful, utilization of triticale as a bridge to enhance recombination rates in wheat could be a useful breeding strategy.

publication date

  • July 2019