A mapping population F2-8 derived recombinant inbred lines (RILs) from a cross between a heat tolerant parent (Cham8) and a drought tolerant parent (Cham6). One hundred and sixty one recombinant inbred lines (RILs) of bread wheat [Triticum aestivum (L.)] with their parents and some local and Australian checks were grown under field conditions at different four heat stressed environments at Sudan and Syria seasons 2009, 2010 and 2011. The objective of the experiment was to assess the performance of the RILS and to dissect their genetic and physiological response under diverse heat stress environments. The phenotypic and genotypic from the F2:8 RILs data obtained from simple sequence repeat (SSRs) and Diversity Arrays Technology (DArT) markers were merged in the QTL mapping. To identify a QTL to be putative a LOD score of 2.5 was selected and an interval distance of 0-25/30cM was determined to pin a marker to represent a QTL. A total number of 72 putative QTLs were identified from the combined environment means. Six QTLs were pinned by (SSR) markers whereas the remaining ones were estimated for DArT ones. These QTL were distributed over 68 linkage groups over the three genomes of the hexaploid wheat. A number of QTLs were found to be associated with more than one trait on different chromosomes, suggesting that these traits could be controlled by a single gene indicating pleiotrpic effect. Those traits have a high phenotypic correlation among them. QTLs for yield and its components were localized in several linkage groups identified on chromosomes 1A, 4A, 2B, 5B, 6B, 7B, 1D, 2D, 3D. The QTLs associated with higher grain yield could be used by breeders as MAS tool to increase the efficiency of conventional breeding.