Wheat is very sensitive to temperature changes. Specifically, processes during wheat leaf, flower, and seed development and photosynthesis, which all contribute to the yield of this crop, are affected by high temperature. While this has to some extent been investigated on physiological, developmental, and molecular levels, very little is known about early signaling events associated with an increase in temperature. Phosphorylation-mediated signaling mechanisms, which are quick and dynamic, are associated with plant growth and development, also under abiotic stress conditions. Therefore, we probed the impact of a short-term and mild increase in temperature on the wheat leaf, spikelet and anther phosphoproteome. The resulting data set provides the scientific community with a first large-scale plant phosphoproteome under the control of higher ambient temperature (Vu, Zhu et al, 2018 J Exp Bot 69:4609-4624; unpublished results). Our analyses also revealed a core set of common proteins between leaf and spikelet, suggesting some level of conserved regulatory mechanisms. Furthermore, we observed temperature-regulated interconversion of phosphoforms, which probably impacts protein activity. Our approach allows gaining insight in signaling events associated with abiotic stress responses, in this case high temperature. Here, I will present our latest results on early signaling events associated with high temperature in wheat, and our progress on using this information as a source for novel breeding markers.