Bread wheat is one of the most important and cultivated food crops worldwide, essential to ensure future food security in view of increasing global population. Identification of quantitative trait loci (QTL) for yield-related traits and closely linked molecular markers are fundamental in order to identify genomic regions for marker assisted selection (MAS) in wheat breeding. A recombinant inbred line (RIL) population, obtained by crossing two bread wheat cultivars (‘Agadir’ and ‘Artico’) was analysed in three different environments. The aim of this work is: i) Construct a high-density linkage map in bread wheat, ii) Perform QTL analysis in order to identify genomic regions involved in yield-related traits, iii) Compare identified regions with previous known regions, iv) Identify promising bread wheat lines for grain yield. The resulting high-density genetic map spans for 2447 cM showing a marker density of 0.41 cM/marker. Sixteen QTLs were detected for glaucosity, spike length, number of seeds, plant height, growth habit, heading date, thousand kernels weight, awn development and colour of seeds on chromosomes 2B, 2D, 4A, 4B, 5A, 7B. Three new regions were identified in this work for growth habit and colour of seeds, while thirteen QTLs were consistently detected in previous studies and some of them coincide with well-known region or cloned genes used in wheat breeding (Ppd, Rht1, Rht8, Vrn3, B1). Finally, six RILs had been identified for favorable traits exploitable for future wheat breeding programs.