By adding two different wheat-tufted wheat 6v#2s 6Al and 6v#4s 6DL translocation chromosome was introduced into the genetic background of csph1b, and a new type of 6v#4s was screened from its separated offspring The transmission rate of 6Al translocation chromosome was higher than that of the original 6v#4s 6DL chromosome increased. However, the formation mechanism of translocation and the exact composition of translocation chromosomes need further analysis. These translocation lines were identified by wheat 55K SNP chip. The results of microarray analysis of three individual plants from 19em2 showed that a large number of Na (accounting for 49.2% of the total number of probes) were found in the 6A chromosomes of the three individual plants. Unexpectedly, they were mainly distributed in the range of 5-596.6mb, accounting for 95.7% of the total 618mb length of the 6A chromosome. It clearly showed that the Na region was not limited to 6As as as expected; However, Gish identification of 19EM2-21-2 and 19EM2-7-22 clearly showed that the translocation chromosome was indeed an inter arm translocation type; The results of fish on 19EM2-21-2 showed that the arm end of wheat chromosome involved in translocation was missing the 6DL specific fluorescence hybridization signal, but it seemed that there was a 6DL specific hybridization signal band near the centromere. The specific PCR marker at the end of the long arm of 6Al proved that both 19EM2-21-2 and 19EM2-7-22 could amplify the specific band of 6Al. It is speculated that the translocation may occur in the Robertsonian translocation between 6DL and 6Al rather than between centromeres.