Psathyrostachys huashanica Keng. (2n = 2x = 14, NsNs), a perennial cross-pollinating plant in the family Poaceae, subtribe Triticeae, possesses many beneficial agronomic traits such as early maturation, high tolerance to abiotic stress, and resistance to yellow rust, take-all fungus and powdery mildew. In this study, a novel wheat-P. huashanica alien disomic addition line 18H28 were identified in the progenies of common wheat line 7,182 crossed with P. huashanica. Chromosome analysis at mitosis and meiosis showed that 18H28 had a chromosome karyotype of 2n = 44 = 22II. Sequential GISH-FISH analysis indicated that 18H28 contained 42 wheat chromosomes plus a pair of P. huashanica chromosomes, and no specific FISH-bands showing on Ns chromosomes with the probe of Oligo-pTa535 and Oligo-pSc119.2. Analysis with expressed sequence tag-sequence tagged sites showed that the added Ns chromosomes in 18H28 were belonging to homeologous group 2. Agronomic trait evaluations showed that 18H28 was much improved in terms of the 1000-grain weight and grain size, and showed high resistance to yellow rust. The addition line could be used as a novel germplasm line for genetic improvement of wheat yield and disease resistance in breeding programs.