The cloning and application of A. cristatum 6P stripe rust resistance genes Abstract uri icon

abstract

  • Agropyron cristatum (2n = 4x = 28, PPPP) is an important relative of common wheat and carries many desirable agronomic traits. Wheat stripe rust, one of the most devastating and widespread diseases of wheat around the world, was caused by Puccinia striiformis f. sp. tritici (Pst). In previous reports, the wheat-A. cristatum 6P translocation line WAT655 carrying 6PS 0.81-1.00 segment was highly resistant to the mixed Pst races of CYR32 and CYR33. In this study, the leaves of the translocation line WAT655 and the recurrent parent Fukuhokomugi (Fukuho) at different time points after Pst inoculation were used for RNA sequencing. The integrated reference sequence of IWGSC RefSeq v1.0 and the A. cristatum “Z559” genome was utilized to analyze the differential analysis of RNA-Seq data, and the Real-Time PCR verification revealed that 6 A. cristatum genes associated with disease resistance exhibited differential expression between the translocation line WAT655 and common wheat Fukuho. Furthermore, the genomic entire sequences of three candidate stripe rust resistance genes (Agr6971, Agr4080, and Agr8173) were used for the transgenic verification, and the three genes can confer high or moderate resistance to the Pst race CYR32 in common wheat. Besides that, the specific molecular markers and the FISH probes of three genes were used for tracing the A. cristatum P genomic components of wheat-A. cristatum breeding lines and the breeding lines that carried three genes and exhibited resistance to the mixed Pst race CYR32 and CYR33 were acquired. Therefore, this study not only provided new exogenous stripe rust resistance genes from A. cristatum for wheat breeding but also provided new breeding materials for the application of these stripe rust resistance genes.

publication date

  • September 2022