Wheat (Triticum aestivum L.), as an important food source of human beings, is one of the most important crops worldwide. The thousand grain weight (TGW) is one of the crucial components of wheat yield and serves as an important means for increasing wheat grain yield in the breeding process. However, the molecular basis of QTL/genes in the regulation of grain weight is still largely unknown. Here, quantitative trait loci (QTL) analysis of TGW was performed in a recombinant inbred line (RIL) population derived from two wheat genotypes differing in grain weight. A total of 4 QTLs controlling TGW were detected across three chromosomes. The QTL of interest on chromosome 2B, which was flanked by wheat 90K markers Ku_c3479_1035 and Jagger_c7991_95 and designated as QTgw-2B, was repeatedly identified in two different environments. We confirmed the presence of QTgw-2B was located in a physical interval from 743.33 Mb to 749.13 Mb in the IWGSC RefSeq v1.0. The 5.8Mb interval region was examined for candidate genes, facilitated by the wheat reference genome annotation information (IWGSC RefSeq v1.1). In total, 75 genes with known function were found. The identification of the target regions as well as the prediction of candidate genes are likely useful for developing effective markers in marker-assisted selection process, and ultimately cloning the causal gene to elucidate the mechanisms involved in wheat TGW.