Plant height is an important factor affecting wheat yield, dwarf and semi-dwarf genes significantly increased wheat yield by reducing plant height and enhancing lodging resistance. Rht4, a gibberellin-responsive (GAR) and recessive dwarfing gene from the x-ray-induced wheat mutant Burt, had no negative effects on coleoptile length and seedling vigor, was located in the chromosome 2BL and linked to SSR marker Xwm317. In this study, Rht4 markedly decreased plant height by 21.29%-24.35%. Combining the analysis of 660K SNP chip and whole genome re-sequencing, 8 KASP markers were developed, Rht4 was mapped to a 9.057 Mb region between AX109817336 and AX-110969031 in chromosome 2BL. Combining the bulked segregant RNA-Seq (BSR-Seq) analysis of the segregating population in two years, there were 10 differential expression genes in the mapping region, two candidate genes (one encodes serine/threonine protein kinase, another encodes disease resistance protein in plants) had large fragment deletions in Burt ert937 mutant and dwarf lines through the whole genome re-sequencing analysis, the genomic sequence amplification and CDS amplification confirmed the deletion. Further works is going on to understand the mechanism of Rht4 on regulating plant development, which will promote the functional characterization of Rht4 dwarfing mechanism and its rational utilization for bread wheat improvement.