In plants, ubiquitin-proteasome system (UPS) and autophagy are the two majors pathways utilized to degrade misfolded or redundant proteins thus maintaining homeostasis during response to abiotic and biotic stresses in plant systems. In African wild rice (Oryza glaberrima), the gene OgTT1 (thermo-tolerance 1 gene) present in chromosome 3 that encode α2 proteasome subunit has been shown to play an important role during response to abiotic stresses, since loss of function of this gene was shown to result in increased sensitivity to heat. Using cDNA and protein sequences of OgTT1 gene as reference, we identified true orthologs of OgTT1 gene in common wheat and its diploid and tetraploid relative species. In different species, the sequence similarity at cDNA level ranged from 71.81% to 89.6%, at CDS level ranged from 87.25% to 89.57%, protein level ranged from 91.45% to 98.27% and at gene level was ranged from (from 36.12% to 46.6%) is lower compare to the similarity at sequence level In TT1 proteins from different species used, three of the six distinct motifs were novel. The proteins were characterized by a single conserved domain belonging to Ntn hydrolase (N-terminal nucleophile) superfamily (Proteasome α2 type) present in all the species. In silico expression analysis indicated that TaTT1 genes were highly expressed in root, leaf and grain under normal condition. The qRT-PCR results suggested that TaTT1 genes were upregulated (>2-fold) under 6h heat stress in wheat seedlings of two heat tolerant genotypes (Giza168 and IC2538749) relative to that in two sensitive genotypes (PBW343 and HD2967). Particularly the TaTT1-3D gene copy highly expressed (FC>50) under 6h heat stress condition. The study thus provided evidence for conserved nature of TT1 gene in bread wheat along with its related species and its role in heat tolerance in bread wheat.