Discovery of new genes for powdery mildew resistance is a constant task for wheat breeders because of the co-evolution of host resistance genes and pathogen avirulent genes. The einkorn wheat accession TA2032 showed resistance against all 24-tested Blumeria graminis f. sp. tritici (Bgt) isolates. Inheritance studies using a recombinant inbred line (RIL), derived from the cross of TA2032 and a susceptible einkorn accession M389, indicated that three powdery mildew resistance genes in TA2032 were responsible for powdery mildew resistance. Molecular marker mapping showed that the three Pm genes were mapped on chromosomes 3AS, 5AL and 7AL, respectively, of which the 3AS gene was at an un-reported locus. This 3AS gene was delimited to a 2.48 cM interval and co-segregated with Xwgrc4717 using 498 F2 plants developed from a RIL that carried only the 3AS gene. A resistance spectrum analysis suggested that pyramiding of the three Pm genes conferred the broad-spectrum resistance.