Wheat is one of the most important food crops in the world. Ear length, ear neck length and sub-ear node length are significantly correlated with yield. At present, there has been great progress in QTL mapping and marker development for ear length and other related traits, but there is still a big gap in the development of effective loci and marker utilization. Therefore, in this study, 164 F6 RIL populations were constructed with Avocet as female parent and Chilero as male parent, and 130 natural populations were used as subjects. A genome-wide genetic map was constructed with 892 markers and a total genetic length of 3,931.14 cM. Inclusive composite interval mapping (ICIM) was used to map four QTL loci for spike neck length on chromosomes 4A, 4D, 5A and 6D, contributing 6.80%-9.15%. Three SUBspike node length QTL locis were detected on chromosomes 6D and 2D, contributing 4.41%-11.26%. Two candidate genes were screened in QNl. Haust-4al, which play an important role in plant growth and vegetative growth. Five candidate genes were screened for QIl. Haust-6dl2, including three leucine-rich repeat proteins, auxin response protein and growth regulatory factor.

QNl.haust-4AL and QIl.haust-6DL were used to develop corresponding KASP molecular markers, and 130 cultivars were tested. Analysis of genotypic and field phenotypic data showed that QNl.haust-4AL and QIl.haust-6DL. The main effect in QNl.haust-4 and QIl.haust-6DL for controlling Long neck and peduncle length the KASP molecular marker developed in this study can be used for gene cloning and marker-assisted breeding of this trait to speed up the breeding process.