Triticum urartu (Tu, 2n = 2x =14, AA) is an important diploid wheat that donated the A genome to tetraploid and hexaploid wheat species. Tu accessions, widely distributed in the countries located in the Fertile Crescent, constitute a valuable primary gene pool for wheat improvement. Further to the publication of draft and fine genome sequence for the Tu accession G1812, we have now constructed a gapless genome assembly for the Tu accession PI428322 originated from Lebanon. By integrating the sequence information generated with HiFi PacBio, Hi-C, and BioNano technologies, we obtained 2,712 contigs, with a contig N50 of 234.02 Mb. The size of the assembled genome was 4.91 Gb, with the seven assembled chromosomes (1A-7A) represented by only 42 contigs. The BUSCO and LAI scores of the assembly were 96.9% and 18.39%, respectively, demonstrating its completeness in both genic and intergenic spaces. Chromosomes 4A and 6A showed the best assembled sequence continuity, with only two gaps in each. The centromeres of five chromosomes (1A, 2A, 3A, 6A, and 7A) were assembled gaplessly. Except for 2A and 5A, the remaining five chromosomes all had telomeres assembled in the termini of short and long arms. The Tu accessions collected from the Fertile Crescent countries were analyzed using the gapless genome of PI428322 as a reference, which have yielded new insights into the mechanism underlying population differentiation and the genes regulating important biological traits in Tu.