With the release of high quality wheat reference genome, the accuracy of exome capture has been enhanced, which makes it to be a new strategy and are accelerating target gene mapping. In order to using the new strategy to map genes rapidly, it needs abundant germplasm with diversified phenotypes. We have established a wheat mutant resource platform, and it contains an exonic TILLING database and thousands of stable mutant lines with broad spectrum mutations. We used a hexaploidy wheat cultivar Jing 411 as wild type. Jing 411 was a core parent in the Northern Winter Wheat Region in China, and its seeds were treated by 12C ion beams, ɣ rays, or ethyl methanesulfonate mutagens, and the M2 generation was captured by exome sequencing. The TILLING database covered over 95% annotated genes of Chinese Spring genome, mutated alleles could be accessed through website http://jing411.molbreeding.com/#/, and could be used for gene functional verification. Stable mutants with phenotypic variations have been identified, and some of the mutants have been used for gene/allele mining on target traits. For example, Rht8 and one of its new allele were cloned by map-based cloning, gene controlling apical spikelet degeneration and QTLs for plant height have been mapped, alleles on thousand grain weight, flag leaf angle or tolerant to low nitrogen have been identified through association analysis.