Common wheat landrace Kaixian-luohanmai carries a gene(s) that promotes homoeologous chromosome pairing in its polyhaploid hybrids with related species. A major QTL responsible for this effect has been mapped to chromosome arm 3AL. However, it is still unknown whether it can be used to induce homoeologous recombination in hexaploid wheat background. In present study, a wheat-Aegilops variabilis 2Sv/2B substitution lines was crossed and backcrossed to Kaixian-luohanmai. Thirteen 2Sv/2B double monosomic lines (BC1F1) were identified and self-pollinated to generate a BC1F2 population. In total, 2210 BC1F2 plants were screened by seven 2Sv/2B specific KASP markers. Forty six 2Sv/2B recombinants were identified, suggesting the frequency of recombination of 2.08%. Some recombinants were further confirmed by the 55K SNP array and in situ hybridization. Similar scenario was also applied to test the 2D/2Sv and 2BL/2RL homoeologous pairs. A very low recombination frequency of 0.13% (1/792) was observed for the former. No recombinants was confirmed between 2RL/2BL although pairing at metaphase I during meiosis was observed. It seems that Kaixian-luohanmai is effectiveness in inducing recombination of homoelogous chromosome pairs which are closely related. Thus, Kaixian-luohanmai can be served as a new tool in chromosome engineering for developing wheat-alien translocation.