Modern wheat breeding programs aim to create cultivars with high genetic potential for grain yield and high bread making quality. Nitrogen fertilization (N) increases grain yield and quality, but also represents a significant production cost and has a negative impact on the environment. The aim of this study was to analyze the effect of year (Y), cultivar (C) and fertilization with nitrogen fertilizer (N) on the grain yield and quality of winter wheat during a three-year study. The experiments were carried out at the location Staro Topolje and included three different treatments with nitrogen fertilizers in the amount of 100 (N100), 140 (N140) and 180 (N180) Kg of applied nitrogen per hectare. For grain yield (GY) and grain protein content (GPC) significant effects of the year, cultivar and treatment with nitrogen fertilizer were determined. For both properties the largest effect had the year (GY-53%, GPC-58%), then treatment with nitrogen fertilizer (GY-5%, GPC-17%) and the cultivar had the smallest effect (GY-4%, GPC-8%). Significant effects of year and cultivar were also found for test weight (TW) and thousand kernel weight (TKW), while treatment with nitrogen fertilizer did not significantly effect for this traits, while the effect of year being considerably higher (TW-80%; TKW-64%) as compared to the effect of cultivar (TW-11%; TKW-24%). Interactions Y × C and Y × N were significant for all traits, but with a small share of the total sum of squares (less than 13%). The C × N interaction, as well as Y × C × N, were not significant for either trait. It was also observed that all tested cultivars responded equally to nitrogen fertilizer treatment for all tested traits. The average of grain yield (GY) and grain protein content (GPC) at the lowest (N100) amount of applied nitrogen fertilizer was 5.92 t/ha; 11.08%, at the medium (N140) 6.56 t/ha; 11.95%, and at the highest (N180) 6,96 t/ha; 12.89%. The great effect of the year on all tested traits indicates the importance of multiannual testing to obtain more precise and better quality results when testing these specified traits.