Efficiency seed production technology system and cost-effective hybrids are the prerequisite for the application of hybrid wheat in production. Sterile line propagation is an indispensable step in the utilization of three-line hybrid wheat, which will inevitably increase seed production cost. In this study, wheat AL-type male sterile lines AL36A (BC12) and Kendong 002A (BC9) were used as test materials. Hormones such as IAA + percolation additives, NAA + percolation additives and JA + percolation additives were sprayed at the anther connective stage and meiosis tetrad stage, and water was sprayed as control. After heading, artificial bagging was carried out, and the seed setting rate of self-crossing was investigated in laboratory. The experiment was carried out in Xinjiang Academy of Agricultural and Reclamation Sciences from 2020 to 2021, and was repeated twice in sequence. In 2020, a total of 5 experimental treatments appeared self-crossing, and the average number of seeds per panicle varied from 7.02 to 54.56. Among them, the average number of seeds per panicle when sprayed with 200 mg/L NAA + percolation additives was 54.56; The average number of seeds per panicle with 150 mg/L JA + percolation additives was 42.21. In 2021, a total of 3 experimental treatments appeared self-crossing, and the average number of seeds per panicle varied from 37.00 to 60.30. Among them, the average number of seeds per panicle when sprayed with 50 mg/L JA + percolation additives was 60.30; the average number of seeds per panicle with 250 mg/L NAA + percolation additives was 46.00. In the above two years, the self-fertilization of the sterile lines by sprayed water and bagging was 0. The results showed that spraying of exogenous hormones could induce wheat cytoplasmic male sterile lines to self-fertilize. However, the reproducibility of the same treatments between different years was poor. In the future, the molecular mechanism of exogenous hormone-induced cytoplasmic male sterile line to become fertile will be studied.