Stripe rust is one of the most devastating diseases of wheat (Triticum aestivum) worldwide. Adult-plant resistance (APR) is an efficient approach to provide long-term protection of wheat from the disease. Lanhangxuan 121 (LHX121) is a commercial cultivar developed from the space mutant of CIMMYT-derived advanced line 92-47. 92-47 has displayed “slow rusting” ever since it was selected from the cross by local cultivar and CIMMYT germplasm. Lanhangxuan 121 showed higher resistance than 92-47 in multiple field environments, however, little is known on the genetic basis of resistance to Pst races. Comprehensive dissection of their genetic variations of resistance will provide insight into the resistance mechanism and facilitate better application of this resistance in breeding. Hence, the main objective of our research was to map and compare the genes/QTL underlying basal resistance in wheat 92-47 and LHX121 using two genetic populations. Two hundred and ninety-eight F2:3 lines were developed from a cross between LHX121 and susceptible cultivar Xinong 822 (XN822). The lines were evaluated for disease severity (DS) in three sites during the 2018-2020 cropping seasons. Affymetrix 660K SNP (single nucleotide polymorphism) chip were used to genotype the lines and their parents. Inclusive composite interval mapping detected three QTL, namely QYrLHX.nwafu-2BS, QYrLHX.nwafu-3BS and QYrLHX.nwafu-5BS, conferring resistance to stripe rust in all tested environments. QYrLHX.nwafu-5BS was mapped to a 7.08 cM interval and explained 9.59%-22.89% of phenotypic variation. Compared with stripe rust resistance genes previously mapped to chromosome 5B, QYrLHX.nwafu-5BS is likely a new APR gene to stripe rust. The other two QTLs were likely to be Yr27 and Yr30 compared with previous studies and based on its pedigree information. The interactions among these genes/QTLs were mostly additive suggesting their potential value in enhancing stripe rust resistance breeding materials as observed in the resistant parent.