The 14-3-3 proteins play vital roles in the regulation of plant growth, development, and responses to stress stimuli. However, the functions of 14-3-3s in response to drought and salt stresses remained unclear in the hexaploid wheat (Triticum aestivum L.), a globally important staple crop. In the present study, a 14-3-3 gene from wheat, designated TaGF14g, was cloned and characterized. Quantitative real-time PCR (qRT-PCR) analyses of 2-week-old plants of the wheat cultivar Chinese Spring showed that TaGF14g was upregulated under the treatments of polyethylene glycol 6000, sodium chloride, hydrogen peroxide, and abscisic acid (ABA). TaGF14g was localized in both cytoplasm and nucleus. Overexpression of TaGF14g in tobacco enhances the tolerance to drought and salt stresses in the transgenic plants. Transgenic tobacco plants had better growth status, longer roots, and relatively higher water contents and survival rates than those of the control plants under drought and salt stresses. Moreover, TaGF14g expression significantly enhanced the reactive oxygen species (ROS) scavenging system to ameliorate oxidative damages to cells. Several known stress-responsive genes in tobacco that are involved in the ABA-signaling pathway, ROS -scavenging system and the ion transporters were all up-regulated in the TaGF14g overexpressing plants as compared with wildtype. Taken together, these results demonstrate that TaGF14g overexpression alters ABA signaling, enhances the physiological processes involved in stress responses (such as the ROS scavenging system), and confers better tolerance to multiple abiotic stresses. This information will be useful for detailed functional analysis of TaGF14g in future studies and for wheat breeding with the aim of stress tolerance enhancement.