Salt stress is one of the major leading threats affecting wheat plant growth and development as salinization of cultivated land increases globally. In order to return from a stressful environment, it is urged to use such strategies through which maximum crop stand could be achieved under saline conditions. Therefore, the proposed study aims to identify novel germplasm in exotic cereal landraces with high salt tolerance by using different approaches. The first study elucidates identifying novel salt-tolerant germplasm from a very large diverse pool (four hundred accessions of different origin) at 200 mM NaCl using fast and efficient physiologically-based screens in hydroponic culture. Twenty genotypes (25 salt-tolerant and 15 salt-sensitive genotypes) out of 400 were selected based on Na+ exclusion in the leaf blade. Selected wheat lines from hydroponic experiment 1 were evaluated hydroponically at different salinity levels (0, 100, 200 mM NaCl). After smart secerned from hydroponic studies, fourteen salt-tolerant, four salt-sensitive, and two check LU26S, Kharchia65 were further tested in pots and saline-sodic field to explore physiological mechanisms of salt tolerance in selected genotypes. Among low Na+ accumulators, V-03094, V0005, V-04178, and V-05121 genotypes gave maximum seed yield in saline soil which were highly linked with higher K+ accumulation and better biochemical and gas exchange attributes. After very smart selection from hydroponic, pot, and field studies, it concluded that V-02156, V-03094, V0005, TURACO, PVN identified as the best Na+ excluders genotypes had better performance with improved physiological and yield attributes in salt stress which can be used in breeding programs to introduce the low Na+ trait in commercial hexaploid wheat cultivars.