VQ proteins are a class of plant-specific proteins containing the conserved motif FxxhVQxhTG (h denotes hydrophobic residues and x represents any amino acid) and are named VQ for the V and Q residues. Accumulated evidence has highlighted the importance of VQ proteins mainly participating in signal pathways through interacting with partners (eg. WRKYs and MAPKs) to regulate plant growth and development and respond to biotic and abiotic stresses. Sharp eyespot, caused by the necrotrophic fungal pathogen Rhizoctonia cerealis, is a devastating disease of bread wheat. However, the role of VQ proteins on wheat Sharp eyespot is not clear. In this study, we identified a VQ gene (named TaVQ) from the wheat Sharp eyespot insensitive material H83, which resulted in enhanced resistance to Sharp eyespot in wheat after gene silencing of TaVQ through virus-induced gene silencing. In addition, we found an interaction between TaWRKY and TaVQ, TaVQ and TaWRKY formed a complex in the nucleus. Yeast two-hybrid experiments showed that the VQ motif of TaVQ is the key to their interaction. In conclusion, our findings provide evidence for the interaction of VQ and WRKY transcription factors in wheat, and suggest that TaVQ negatively regulates wheat resistance to Rhizoctonia cerealis through the interaction with TaWRKY.