Global wheat yields were worth US$145 billion during 2015-2016, and it is grown in diverse areas ranging from 45°S in Argentina to 67°N in Russia. In the US southeast (SE), soft red winter wheat is the leading wheat class grown mainly for crackers, flatbreads, and pastries. Hessian fly (HF) is one of the major biotic stresses for wheat that decreased harvested acres in the state of Georgia (GA) from 145,000 to 60,000 from 2015 to 2019. In addition to GA, this pest has led to millions of dollars in losses for wheat farmers in other southern states. For instance, it caused total crop loss during 2007 for many farmers in north central Oklahoma. Genetic resistance is the most cost-effective solution for managing HF infestation. HF susceptible line AGS 2038 and resistant line UGA 111729 were crossed to generate an F6 population of 225 recombinant inbred lines (RIL). The RIL population was phenotyped in field (F) trials in Williamson and Plains, GA in the 2020-2021 growing season. It was also evaluated in growth chamber (GC) studies in 2019 and 2021 seasons. Using inclusive composite interval mapping analysis for the percentage of resistant plants per line, number of pupae and larvae per sample, and number of infested tillers; a major QTL was detected on the long arm of chromosome 3D, which accounted for 11.32% (F) and 45.89% (GC) total phenotypic variance. This QTL consisted of a region of 9.86 cM. Marker IWB65911, co-localizing with resistance gene H32, was associated with this QTL peak, which had LOD scores of 14.98 (F) and 62.22 (GC). These findings demonstrated that UGA 111729 may have resistance gene H32 instead of H24, which has been reported frequently since the 1990s in the progenitor of UGA 111729, KS89WGRC06. H32 KASP markers will be conducted on UGA 111729 and AGS 2038 for confirmation.