Global warming expands the arid land in the world, and drought stress is one of the environmental factors that dramatically reduces agricultural productivity. Of the three major crops, wheat is widely cultivated in semi-arid regions, and its productivity is often affected by drought. Improving the drought stress tolerance of wheat is essential for increasing food supply. In previous studies, we developed TaPYLox wheat that is overexpressing the phytohormone abscisic acid (ABA) receptor, which is a key gene for the drought stress response. TaPYLox has acquired both drought stress tolerance and water-saving trait, which enables efficient grain production with less water. In this study, TaPYLox was used to identify ABA-dependent and ABA-independent drought stress-responsive gene expression and metabolites. In this presentation, we would like to link the relationship between gene expression and metabolite, understand the drought stress response in wheat at the molecular level, and discuss how they can be used for wheat breeding.