To study the gene aggregation of FHB resistance in the descendants of Nankang 1 and Shannong 102, screen out wheat plants with good FHB resistance, and cultivate wheat varieties with FHB resistance. The resistance to FHB resistance of 74 wheat strains under the greenhouse environment and 185 wheat strains under the net room environment was identified by single-flower instillation, and the resistance of each wheat plant was combined with the molecular markers of the FHB resistance gene. The type of resistance gene combination is determined. Among the 74 wheat materials grown in the green house environment, 6 high-resistance materials and 20 medium-resistance materials were initially screened; while in the net room environment, 28 high-resistance materials and 43 medium-resistance materials were selected from 185 wheat materials. No immune wheat was found. When containing one single resistance gene, the plants with Fhb1 showed better resistance than those with other genes. When having two resistance genes, the plants with the combination of Fhb1 + Fhb4 and Fhb2 + Fhb4 showed better resistance to FHB than those with other combinations. However, Fhb2 + Fhb5 and Fhb4 + Fhb5, two combinations containing Fhb5, have a weak ability to improve disease resistance. In three resistance genes combination, the materials with Fhb1 genes performed significant resistance to the materials without Fhb1 gene. The plants containing four resistance genes showed significant resistance both in two environments. This experiment once again proved that Fhb1 is currently the best FHB resistance gene, and there are differences in FHB resistance between different gene polymerizations.