Bread wheat is one of the world's most significant staple food crop. It is grown on 20% of the world's agricultural land and is the primary source of food for 40% of the world's people. Wheat production can be hampered by a variety of biotic causes, including the plant-parasitic nematode (PPNs). PPNs cause annual damages of up to $157 billion (Chariou and Steinmetz, 2017). Root lesion nematodes (RLNs) and cereal cyst nematodes (CCNs) are economically important plant parasitic nematode species, responsible for significant damage to wheat production. Although, there are variety of cultural practices available for managing these nematodes, using resistant and tolerant wheat varieties is the most successful, cost-effective, and ecologically friendly approach. Genome wide association study (GWAS) is an alternative approach to quantitative trait loci (QTL) that do not require the development of bi-parental mapping population and show a higher mapping resolution investigating many more meiotic recombination events. In the present study, single locus and multi locus methods (CMLM, FarmCPU, MLMM, BLINK, etc.) were used for GWAS analysis. Significant marker traits associations (MTAs) were observed after GWAS analysis on different chromosomes. These associated markers with important MTAs can be further utilized for wheat improvement through various molecular breeding approaches.