Durum wheat (Triticum turgidum subsp. Durum (Desf.) Husnot) is a vital cereal species, mainly grown under rainfed conditions in the Mediterranean area. However, this crop is affected by various biotic and abiotic stress accented by climate change circumstances. Hessian fly is a serious pest of durum wheat worldwide; yield losses can reach up to 32%; besides, this pest evolves rapidly under intense selection pressure to overcome resistance. Thus, to ensure the availability of resistance sources, the diversity of «CerealMed» durum wheat collection, derived from the Global Durum wheat Panel, was assessed for Hessian fly resistance in greenhouse conditions. Phenotypic scoring for this insect pest was performed 20-25 days after infestation based on symptoms of attacks, number of dead larvae, and flaxseeds in each seedling. Out of the 312 genotypes screened, 12 have been identified as highly resistant to Hessian fly. The selected accessions could be used in breeding programs to develop improved durum wheat varieties adapted to Mediterranean environments with enhanced resistance to Hessian fly.