Nitric oxide (NO) is essential molecule that regulates a wide range of growth and developmental processes through numerous regulatory systems. Earlier we have revealed a pronounced growth stimulating effect of nitric oxide (NO) on wheat plants (Triticum aestivum L.), and these data point towards the active influence of NO on protein metabolism. We have carried out the proteomic analysis of proteins in response to sodium nitroprusside (SNP), the NO donor, in wheat seedlings. Analysis of proteins by two-dimensional electrophoresis has revealed that SNP caused the promotion of protein metabolism, and this was evidenced by an intensification of protein signals in a wide range of molecular weights and isoelectric points. It was found that NO-stimulated growth of wheat plants was accompanied by accumulation of multiple proteins. The obtained data suggests an important role of nitric oxide in the activation of protein metabolism which is the basis for stimulation of plant growth.