In wheat genome, we identified RuvBL family of helicase genes, which represent a conserved family of genes, occurring in all organisms ranging from single celled organisms (bacteria, yeast) to multicellular organisms (human, Arabidopsis thaliana, Oryza sativa, Drosophila etc.). Nine wheat RuvBL genes (located on nine different chromosomes) were identified and named as follows: (i) TaRuvBL2a-2A, (ii) TaRuvBL2a-2B, (iii) TaRuvbl2a-2D, (iv) TaRuvBL1b-3A, (v) TaRuvBL1b-3B, (vi) TaRuvbl1b-3D, (vii) TaRuvBL1a-4A, (viii) TaRuvBL1a-4B and (ix) TaRuvBL1a-4D). Structure and function analysis of the genes revealed the following features: (i) Length of the genes ranged from 1647 bp to 2197 bp. (ii) synteny analysis suggested close relationship with corresponding genes in related species including maize, rice, barley and the grass B. distachyon. (iii) Promoter analysis revealed the occurrence of cis-elements, which respond to stimuli like stress, hormones growth and development. (iv) The genes were associated with transposable elements belonging to the following classes: LTR, non-LTR, TIR, non TIR. (v) Two genes, namely TaRuvBL1a-4A, TaRuvBL1a-4B also carried targets for a miRNA, namely tae-miR164. (vi) The gene ontology (GO) analysis revealed that TaRuvBL genes were closely associated with following activities: ATP binding associated with ATP-dependent activity and formation of histone acetyltransferase complex. Similarly, analysis of structure (primary and secondary) and function of proteins and the motifs carried by them had the following attributes: (i) localized mainly in the nucleus and cytoplasm; (ii) the protein encoded by the gene TaRuvBL1a-4A was involved in protein-protein interactions with ten other proteins belonging to same or different protein families; (iii) the phylogenetic analysis revealed that RuvBL was divided into two subdivisions (RuvBL1 and RuvBL2). In silico expression analysis suggested that these genes were differentially expressed under heat/or drought. In future, we may examine the utility of these genes in developing wheat cultivars with heat/drought tolerance.