Wheat is the second largest crop in China food crops in China's agricultural production. Drought is one of the most common environmental threats to plant growth and productivity. Psb28 is a soluble protein in the PSII complex. Existing studies can prove that Psb28 protein can participate in the assembly and repair process of PSII under high temperature stress, but the relevant functions of Psb28 protein are poorly understood.

To study Psb28 might be involved in the response of wheat to drought stress, the Psb28 gene cloned from wheat was constructed by fusion expression vector for the first time, and the subcellular localization of the expressed protein was carried out in this experiment. Subsequently, the overexpression vector of Arabidopsis was constructed and related genetic materials were obtained. The aim of this study was to preliminarily explore the drought resistance mechanism of wheat Psb28 gene by overexpressing the relative expression of genes under drought stress and the physiological indicators of Arabidopsis.

The analysis of protoplast subcellular localization showed that TaPsb28 protein was localized in chloroplast matrix. The overexpressed TaPsb28 transgenic Arabidopsis has showed a higher drought tolerance by a higher water retention capacity under a certain degree of drought. In addition, overexpression of TaPsb28 could reduce the damage of membrane under drought stress. And increased the relative expression of genes under drought stress. TaPsb28 played an active role in the drought resistance of plants. According to the results of this experiment, we found that overexpression of TaPsb28 could decrease water loss rate of leaves by regulating stomatal closure under drought stress, then enhanced the drought resistance of Arabidopsis.