With the aim to introduce translocated genomic segments carrying BNI properties into cultivated wheat varieties, the backcross approach was used, taking chromosome addition lines (CAL) as a donor and recently released Indian varieties as a recipient.

The CAL was developed through the crosses of L. recemosus and T. aestivum due to harbouring BNI quality and other favourable traits for wheat improvement. We validated the molecular markers developed from de Novo sequencing of Leymus species to identify translocated and non-translocated lines of wheat and for detecting the introgressed segment in the backcrossed progenies in different cross combinations.

Molecular markers were utilized to confirm the introgression of the BNI segments instead of GISH. While GISH would be used to verify the translocation region in the genome of introgressed lines.