Email: lihaosheng810@163.com; lgy111@126.com

To analyze gene distribution in wheat lines involved in 2019-2020 Shandong regional trial, one hundred and twenty-six wheat lines were tested with 64 Kompetitive Allele Specific polymerase chain reaction (KASP) markers which were specific to 55 genetic loci. The markers are related to yield, quality, resistance, flower time and etc. Over 90% KASP markers give acceptable genotyping, which provides an efficient technology for gene identification.

Favorable allele frequencies of 20 genes are above 80%, including Vrn-A1, Vrn-B1, Vrn-D1, Ppd-A1, Ppd-B1, Ppd-D1, Psy-D1, Glu-A3g, Rht-D1, Pinb-D1, TaCwi-A1-1, TaGW2-6B, TaSus1-7B, TaGASR7-A1, 1-feh-w3, TaDreb-B1, PRR-A1, PRR-B1, TaFT3-B1 and TaMOT1-D1. Favorable allele frequencies of 26 genes are below 30%, including Vp1-B1, Ppo-D1, TaPds-B1, Zds-A1, TEF-7A, Lr46, Glu-B3g, TaGS5-A1, TaCwi-4A, 1B/1R, Rht-B1, Lr68, TaELF3-B1, Pina-D1, Sbwm1, TaPHS1, Pm21, COMT-3B, TaCKX-D1, TaSdr-B1, Pch1, Lr34, Yr15, Fhb1, TaMoc-7A and TaGS-D1, notably, favorable alleles of TaMoc-7A, Lr34, Fhb1, Pch1, Yr15, and TaSdr-B1 are not detected.

Favorable allele frequencies of 9 genes are between 30% and 80%, including Pinb2-V, TaPod-A1, Lox-B1, Glu-A1, Glu-D1, TaGS2-B1, TaGW2-6A, TaSus1-7A, and Lr14a. Favorable allele frequencies of 55 genes show polarization trend. Among all tested lines, JinongCH03, CG086, and JinongCH01 are the only one possessing favorable alleles of TaCKX-D1, Pm21, and COMT-3B, which could be used as gene donors for kernel weight, powdery mildew resistance, and stem lignin content improvement and breeding.

In this study, genotypes of 55 genes in 126 wheat lines are clarified, allelic distribution of important agronomic genes in wheat lines attending the Shandong regional trial was basically clarified, which will serve as the foundation for marker assistant selection breeding.