The aim of this research was to examine geographical distribution of Ppd and Vrn alleles in diverse set of wheat genotypes. Research was conducted on 120 hexaploid wheat varieties originating from 25 countries (Hungary, Japan, USA, Italy, France, Austria, United Kingdom, Australia, Mexico, Argentina, Canada, Russia, India, Serbia, Chile, North Macedonia, Croatia, Czech Republic, China, Sweden, Germany, Ukraine, Romania, Bulgaria and Slovakia).

For research purposes, varieties were grouped into geographical regions – North America, Central and South America, Asia and Australia, West Europe, East Europe, Central Europe, South Europe, Croatia-Serbia. Genomic DNA was extracted from the young leaves according to CTAB extraction method. PCR analysis was conducted using functional markers specific for Ppd-B1, Ppd-D1, Vrn-A1, Vrn-B1, Vrn-B3 and Vrn-D1 loci.

Amplification products were analyzed using horizontal gel electrophoresis. At Ppd-B1 locus, Ppd-B1b allele prevailed over Ppd-B1a allele in all geographical regions. The highest frequency of Ppd-D1a allele (85 %) was recorded in varieties from South Europe, while the highest frequency of Ppd-D1b allele (75 %) was recorded in varieties from North America.

The highest frequency of dominant Vrn-A1a allele (50 %) and Vrn-A1b/c allele (25 %) was observed in varieties from North America. The recessive vrn-A1 allele was the most abundant in varieties from South Europe (100 %). At Vrn-B1, Vrn-B3 and Vrn-D1 loci recessive alleles prevailed over dominant alleles in all geographical regions.

The lowest frequency of vrn-B1 allele was observed in varieties from Central and South America, and the lowest frequency of vrn-D1 allele in varieties from Asia and Australia.