Huijun Guo: guohuijun@caas.cn, Luxiang Liu: liuluxiang@caas.cn

Mutation induction has been utilized in plant breeding for over 90 years, the heritable changes result in new traits and increase the genetic diversity of crops. With the development of functional genomics, mutants on variable traits have been applied to unveil functions and pathways of target genes in Arabidopsis, rice, maize and other crops.

Wheat functional genes have been uncovered rapidly in recent several years with the release of reference genome and low-cost sequencing technique, therefore it need more diversified mutant resources under multiple genetic background to accelerate gene function characterization and elaboration. We used a hexaploidy winter wheat variety, Jing411, as the wild type, and used gamma rays, carbon ion beams, spaceflight, ethyl methane sulphonate as mutagens, established a mutant resource with broad spectrum phenotypic variations and a mutant library with genotypic variations.

The phenotypic mutant resource was consist of advanced stable lines, which have been used to uncover novel QTLs, genes and/or alleles, such as the semi-dwarf gene Rht8 and its alleles, flag leaf angel gene SPL8 and its alleles, the Q gene and its alleles, mutants on starch and resistant starch etc..

The genotypic mutant resource (http://jing411.molbreeding.com/#/query) contained functional mutations, which covered 97.09% of high confidence genes and 71.12% of low confidence genes. Up-to-date, the mutants have been shared and utilized in wheat community, and over 600 samples have been provided to more than 50 research groups.