Water availability is one of the limiting constraints for wheat production. To alleviate some of the challenges faced by agriculture today, a better understanding of the root architecture of wheat plants are necessary to enhance water use efficiency and fertiliser use. Root angle is an important aspect of wheat root architecture that needs to be clarified. It is suggested that plants with narrow seminal root angles tend to have deeper roots, which might give better access to subsoil moisture. This hypothesis was tested by measuring root angles and examining its relationship with yield and other parameters. A clear-pot-method was initially investigated to determine differences in root architecture between high and low yielding cultivars. Additionally, a modified high-throughput hydroponics system was devised for screening of multiple high yielding entries from various international nurseries. Root lengths and root/leaf biomass of 8-day old seedlings were determined. Root angle was determined on each of the entries using the software ImageJ. Significant differences were observed between low and high yielding cultivars planted in clear pots. Plant height, root length, root and leaf biomass were 23%, 32%, 33% and 28% higher respectively in the high yielding cultivar. Moderate uphill relationships (R2>0.50) were found between root length/root biomass, root length/leaf biomass and root/leaf biomass. A root:shoot ratio >1 was observed in more than 75% of the entries, indicating a greater root density that will consequently ensure greater aboveground biomass. For plants that grow largely on stored soil moisture, a deep root system with a high root:shoot ratio will be an advantage. High levels of genetic variation for seminal root angle were observed. The entries screened in this study could be assigned to three groups, namely narrow, moderate and wide, based on their root angles. Yields varied between 6.8 and 8.8 ton/ha under supplementary irrigation at ARC-Small Grain, Bethlehem, South Africa. Preliminary data from this study of high-yielding potential germplasm suggest that genetic variation exists for root angle. However, no significant correlation could be found between root angle and yield. A possible explanation is that the entries used in this study were specifically selected for their high yields. Root length and biomass are fundamental elements and contributors to the stable expression of high yields in wheat. As yield stability is a complex quantitative genetic trait, influenced by root architecture, minor favourable trends may prove to be significant in future.