In recent years, durum wheat production in North America has been seriously threatened by epidemics of Fusarium head blight (FHB), caused mainly by Fusarium graminearum. Losses due to this disease include yield, test weight, seed quality, food and feed quality and when severe, market access. More importantly, it is the contamination with mycotoxins, such as deoxynivalenol (DON), in Fusarium infected durum kernels that causes the most serious economic as well as food and feed safety concerns. Deployment of FHB-resistant cultivars is considered the most effective and cost-efficient strategy to combat this disease. However, limited progress has been made in improving FHB resistance in durum wheat because there is narrow genetic variation for FHB resistance in elite germplasm. Therefore, combining FHB resistance from different sources and extensive screening of new germplasm collections is important. We have evaluated 330 lines of tetraploid wheat for Type II FHB resistance over two greenhouse seasons. Specifically, we examined breeding lines and several European tetraploid lines that carrying the FHB resistance gene Fhb1 introgressed from hexaploid wheat, and lines carrying putative novel sources of resistance. In total we examined 167 breeding lines from Canada, 110 lines and five cultivars from Austria, 15 substitution lines from USA and 10 susceptible and moderately susceptible checks. We pointinoculated spikes with a 3-ADON chemotype of F. graminearum, which is the aggressive chemotype in Western Canada and scored disease severity 21 days after inoculation. Wide variation in severity responses was observed among the evaluated material. Phenotypic expression of resistance in lines carrying Fhb1 was genetic background dependent but on average, a 20% reduction in disease severity relative to moderately susceptible durum check cultivars was observed. About 15% of the breeding lines showed a disease severity of 33% or less. These lines are being used in our intensive breeding efforts to enhance FHB resistance in durum wheat.