Molecular markers can be used in plant breeding for quality assurance (QA) and quality control (QC). QA is important for proactively preventing errors and creating a high-quality product (e.g. a new plant variety), while QC is targeted towards identifying errors in the product (e.g., varietal identity). Thus, QA/QC are essential in deploying high quality seed, an important factor for increasing and stable crop yields per unit area. For QA/QC deployment, a common set of highly polymorphic molecular markers is important to be able to reveal consistent and reproducible results along with minimal costs by batch ordering. We used a diversity set consisting of 1102 different wheat lines genotyped with the Illumina iSelect 90K SNP assay and a large breeding set including 46,089 advanced breeding lines genotyped with Genotyping-bySequencing markers to select our QC marker panel. Based on more than 7 million pair-combinations in power analysis for discrimination ability, we selected 160 genome-wide markers distributed across all three wheat genomes. These markers have been converted to KASP assays and have been used to initially evaluate three defined use cases: (a) the percent purity of advanced lines distributed via international nurseries and yield trials (b) pedigree reconstruction and the identity of advanced lines, (c) the degree of hybridity in hybrid wheat. We will present the usefulness of the QC marker panel and validate the status of seed in the CIMMYT Global Wheat Program.