Yellow or stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of bread wheat (Triticum aestivum L.) causing significant yield losses worldwide. Recent emergence of new virulent races and presence of highly divergent genetic lineages in various geographical regions calls for rapid characterization and deployment of new rust resistance genes. Seedling tests using current Mexican yellow rust (YR) races MEX14.191 and MEX16.04 identified CIMMYT advanced line Huirivis#1 to carry adequate levels of all stage resistance producing seedling IT response of ;1CN1. The parents and F5 RILs (150 lines) derived from the cross of PBW343 (susceptible parent) and Huirivis#1 (resistant parent) were evaluated for seedling reaction to YR using isolate MEX16.04 and genotyped using DArT-Seq markers at CIMMYT. IciMapping was performed using 18K polymorphic SNP markers. The 2BL region flanked by SNP markers 1245237-2B and 5410696-2B showed close genetic association with gene temporarily designated as YrHu explaining 40% of the phenotypic variation. Stripe rust resistance genes Yr5, Yr7, Yr27, Yr31and YrSp have been reported on chromosome 2B, however, Yr5 and YrSp gave IT response 0 and Yr7, Yr27 and Yr31 gave a compatible IT response 4 when tested with the two races suggesting that the gene on 2BL is different. Based on their specificity to YR pathotypes and host reactions in seedling stage suggest that YrHu could be a new resistance gene. Markers flanking 2BS locus will be converted into kompetitive allele specific PCR (KASP) markers for marker-assisted selection.